环境分子识别与传感研究组课题组简介
主要研究方向为生物分析和传感，环境分析，新型分析检测技术和方法的发展与应用，新型亲和生物配体的分子识别和分析分离应用，纳米材料的分析传感应用，环境和健康相关蛋白质与小分子的灵敏分析检测和分离，分子识别与相互作用机制研究
环境分子识别与传感研究组研究队伍
环境分子识别与传感研究组承担项目
- 国家自然科学基金: 基于适配体亲和分析生物毒素分子的研究，2016-2019，主持。
- 国家自然科学基金重点项目：针对蛋白质-核酸相互作用研究的单分子荧光偏振分析方法学与技术，2015-2019，参加。
- 国家自然科学基金优秀青年科学基金项目，分析化学，2013-2015，主持。
环境分子识别与传感研究组近期成果
- Crystal structures of thrombin in complex with chemically modified thrombin DNA aptamers reveal the origins of enhanced affinity, Rafal Dolot, Curtis H. Lam, Malgorzata Sierant, Qiang Zhao, Feng-Wu Liu, Barbara Nawrot, Martin Egli,* Xianbin Yang* Nucleic Acids Research 2018, 46, 4819-4830.
- Competitive Horseradish Peroxidase-Linked Aptamer Assay for Sensitive Detetction of Aflatoxin B1, Linlin Sun, Qiang Zhao*, Talanta, 2018, 179,344-349.
- Enhancing the Affinity of Anti-Human α-Thrombin 15-mer DNA Aptamer and Anti-Immunoglobulin E Aptamer by PolyT Extension, Yunlong Bai, Yapiao Li, Dapeng Zhang, Hailin Wang, Qiang Zhao*, Analytical Chemistry, 2017, 89, 9467-9473.
- Detection of Human Neutrophil Elastase by Aptamer Affinity Capillary Electrophoresis Coupled with Laser-induced Fluorescence Using Specified Site Fluorescently Labeled Aptamer, Yunlong Bai, Hailin Wang, Qiang Zhao*, Analytical Bioanalytical Chemistry , 2017, 409, 6843-6849.
- Investigation of Effects of Metal Ions in Sample Buffer on Capillary Electrophoresis Coupled with Laser-Induced Fluorescence Analysis of Thrombin Using Dye-Labeled 29-mer DNA Aptamer, Yunlong Bai, Qiang Zhao*, Analytical Methods, 2017, 9, 5684-5690.
- Rapid Fluorescent Detection of Immunoglobulin E Using an Aptamer Switch Based on Binding-Induced Pyrene Excimer, Yunlong Bai, Qiang Zhao*, Analytical Methods, 2017, 9, 3962-3967.
- Aptamer based surface plasmon resonance sensor for aflatoxin B1, Linlin Sun, Liqing Wu, Qiang Zhao*, Microchimica Acta, 2017, 84, 2605-2610.
- Competitive fluorescence anisotropy/polarization assay for ATP using aptamer as affinity ligand and dye-labeled ATP as fluorescence tracer, Yapiao Li, Linlin Sun, Qiang Zhao*, Talanta, 2017, 174, 7-13.
- Direct fluorescence anisotropy assay for cocaine using tetramethylrhodamine-labeled aptamer, Yingxiong Liu, Qiang Zhao*, Analytical Bioanalytical Chemistry, 2017, 409, 3993-4000.
- Affinity capillary electrophoresis with laser induced fluorescence detection for thrombin analysis using nuclease-resistant RNA aptamers, Lihua Hao, Yunlong Bai, Hailin Wang, Qiang Zhao* , Journal of Chromatography A, 2016, 1476, 124-129.
- Determination of the platelet-derived growth factor BB by a competitive thrombin-linked aptamer-based Fluorometric assay, Limin Guo, Qiang Zhao*, Microchimica Acta, 2016, 183, 3229-3235.
- An aptamer assay using rolling circle amplification coupled with thrombin catalysis for protein detection, Limin Guo, Lihua Hao, Qiang Zhao*, Analytical Bioanalytical Chemistry, 2016, 408, 4715-4722.
- Thrombin-linked aptamer assay for detection of platelet derived growth factor BB on magnetic beads in a sandwich format, Limin Guo, Qiang Zhao*, Talanta, 2016, 158, 159-164.
- Lihua Hao, Qiang Zhao*, Microplate based assay for thrombin detection using an RNA aptamer as affinity ligand and cleavage of a chromogenic or a fluorogenic peptide substrate, Microchimica Acta, 2016, 183, 1891-1898.
- Y. Liu, Q. Zhao*, Fluorescence anisotropy assay for D-vasopressin with tetramethylrhodamine-labeled aptamer, Anal. Methods, 2016, 8, 2383-2390.
- L. Hao, Q. Zhao*,Using fluoro modified RNA aptamers as affinity ligands on magnetic beads for sensitive thrombin detection through affinity capture and thrombin catalysis, Anal. Methods, 2016, 8, 510-516.
- Q. Zhao*, Q. Lv, H. Wang, Aptamer fluorescence anisotropy sensors for adenosine triphosphate by comprehensive screening tetramethylrhodamine labeled nucleotides, Biosens. Bioelectron., 2015, 70, 188-193.
- L. Hao, Q. Zhao*, A fluorescein labeled aptamer switch for thrombin with fluorescence decrease response, Anal. Methods, 2015, 7, 3888-3892.
- L. Lv, L. Guo, Q. Zhao*, Aptamer and rolling circle amplification-involved sandwich assay for platelet-derived growth factor-BB with absorbance analysis, Anal. Methods, 2015, 7,1855-1859.
- D. Zhang, R. Fu, Q. Zhao, H. Rong, H. Wang*, Nanoparticles-free fluorescence anisotropy amplification assay for detection of RNA nucleotide-cleaving DNAzyme activity, Anal. Chem., 2015, 87, 4903-4909.
- Q. Zhao*, Q. Lv, H. Wang, Identification of allosteric nucleotide sites of tetramethylrhodamine-labeled aptamer for noncompetitive aptamer-based fluorescence anisotropy detection of a small molecule, ochratoxin A, Anal. Chem., 2014, 86, 1238-1245.