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  • Environmental Bio-analytical Chemistry


    The Environmental Bio-analysis group under the direction of Professor Liang-Hong Guo was founded in 2004, with current research interests focused on the molecular toxic mechanisms of new pollutants including nanomaterials and the development of new analytical methodology and instruments for environmental pollutants. In aspect of environmental molecular toxicology, our effort is concentrated on the interactions between new persistent organic pollutants (POPs) such as PBDEs and PFCs and bio-molecules including metabolic enzymes, transporting proteins, and nuclear receptors. The tasks are to reveal the consequence of such interactions on cellular functions and toxic effects, as well as the structural activity relationship of these chemicals. In regard to environmental bio-analysis, we try to develop rapid, ultrasensitive, and high throughput immune assays for quantitative detection of POPs based on fluorescence, electrochemisty, and electrochemiluminescence techniques. The investigation also includes the development of toxic mechanism based toxicity sensing techniques for the rapid screening of carcinogenic and endocrine disrupting chemicals.


    Selected publications

    1. Zhong-Min Li,Liang-Hong Guo* and Xiao-Min Ren. Biotransformation of 8:2 fluorotelomer alcohol by recombinant human cytochrome P450s, human liver microsomes and human liver cytosol. Environmental Science Processes & Impacts.2016, 18, 538-546.
    2. Xiao-Min Ren, Lin-Ying Cao, Jing Zhang, Wei-Ping Qin, Yu Yang, Bin Wan and Liang-Hong Guo*. Investigation of the Binding Interaction of Fatty Acids with Human G protein-Coupled Receptor 40 Using a Site-Specific Fluorescence Probe by Flow Cytometry. Biochemistry. 2016, 55:1989-1996.
    3. Ren XM, Qin WP, Cao LY, Zhang J, Yang Y, Wan B, Guo LH*. Binding Interactions of Perfluoroalkyl Substances with Thyroid Hormone Transport Proteins and Potential Toxicological Implications. Toxicology. 2016,366:32-42.
    4. Ren X, Cao L, Yang Y, Wan B, Wang S, Guo L. In vitro assessment of thyroid hormone receptor activity of four organophosphate esters. J Environ Sci (China). 2016, 45:185-90.
    5. Yu Yang, Qi-Yan Lv, Liang-Hong Guo*, Bin Wan, Xiao-Min Ren, Ya-Li Shi, Ya-Qi Cai. Identification of protein tyrosine phosphatase SHP-2 as a new target of perfluoroalkyl acids in HepG2 cells. Arch. Toxicol., 2016, DOI: 10.1007/s00204-016-1836-2. 
    6. Sufang Wang, Qiyan Lv, Yu Yang*, Liang-Hong Guo*, Bin Wan, Xiaomin Ren, Hui Zhang. Arginine decarboxylase: A novel biological target of mercury compounds identified in PC12 cells. Biochem. Pharmacol., 2016, 118, 109-120.
    7. Hui Zhang, Lixia Zhao, Fanglan Geng, Liang-Hong Guo,* Bin Wan, Yu Yang. Carbon dots decorated graphitic carbon nitride as an efficientmetal-free photocatalyst for phenol degradation. Appl. Catal. B. 2016, 180: 656-662.
    8. Xuejing Cui, Bin Wan*, Liang-Hong Guo*, Yu Yang, and Xiaomin Ren. Insight into the Mechanisms of Combined Toxicity of Single-Walled Carbon Nanotubes and Nickel Ions in Macrophages: Role of P2X7 Receptor. Environ. Sci. Technol. DOI: 10.1021/acs.est.6b03842.
    9. Xuejing Cui, Bin Wan*, Yu Yang, Xiaomin Ren, Liang-Hong Guo*, and Hui Zhang. Crucial Role of P2X7 Receptor in Regulating Exocytosis of Single‐Walled Carbon Nanotubes in Macrophages. Small, DOI:10.1002/smll.201602410.
    10. Jie Hou, Bin Wan*, Yu Yang, Xiao-Min Ren, Liang-Hong Guo*, and Jing-Fu Liu. Biodegradation of Single-Walled Carbon Nanotubes in Macrophages through Respiratory Burst Modulation. International journal of molecular sciences, 2016, 17:409.
    11. Changjiang Hou, Lixia Zhao*, Fanglan Geng, Dan Wang, Liang-Hong Guo*, Donor/acceptor nanopartical pair-based singlet oxygen channeling homogenous chemiluminescence immunoassay for quantitative determination of bisphenol A, Anal. Bioanal. Chem., 2016, DOI: 10.1007/s00216-016-9584-y
    12. Yongguang Yin, Xiaoya Yang, Ligang Hu, Zhiqiang Tan, Lixia Zhao, Zhen Zhang, Jingfu Liu, Guibin Jiang, Superoxide-Mediated extracellular biosynthesis of silver nanoparticles by the fungus fusarium oxysporum, Environ. Sci. Technol. Lett, 2016, 3(4) 160-165.
    13. Hui Zhang, Liang-Hong Guo,* Lixia Zhao, Bin Wan, Yu Yang. Switching Oxygen Reduction Pathway by Exfoliating Graphitic Carbon Nitride for Enhanced Photocatalytic Phenol Degradation. J. Phys. Chem. Lett. 2015, 6: 958−963.
    14. Hui Zhang, Liang-Hong Guo,* Dabin Wang, Lixia Zhao, Bin Wan. Light-Induced Efficient Molecular Oxygen Activation on a Cu(II)-Grafted TiO2/Graphene Photocatalyst for Phenol Degradation. ACS Appl. Mater. Interfaces 2015, 7: 1816−1823.
    15. Ren XM, Zhang YF, Guo LH*, Qin ZF, Lv QY, Zhang LY. Structure-activity relations in binding of perfluoroalkyl compounds to human thyroid hormone T3 receptor. Arch Toxicol. 2015, 89(2):233-42.
    16. Lixia Zhao, Fan Di, Hui Zhang, Bin Wan, Yu Yang, Synergistic induced chemiluminescence of carbon dots by oxidants and strong alkaline solution, Sci. Adv. Today, 2015,1,25216.
    17. Dabin Wang, Lixia Zhao*, Liang-Hong Guo*, Hui Zhang, Bin Wan, Yu Yang, Online Quantification of O2•- and H2O2 and Their Formation Kinetics in Ultraviolet (UV)-Irradiated Nano-TiO2 Suspensions by Continuous Flow Chemiluminescence, ACTA CHIMICA SINICA, 2015, 86(21), 10533-10539.
    18. Jing Sun, Liang-Hong Guo,* Hui Zhang,* Lixia Zhao. UV Irradiation Induced Transformation of TiO2 Nanoparticles in Water: Aggregation and Photoreactivity. Environ. Sci. Technol. 2014, 48: 11962-11968.
    19. Sufang Wang, Bin Wan, Lianying Zhang, Yu Yang*, Liang-Hong Guo*. In vitro inhibition of lysine decarboxylase activity by organophosphate esters. Biochem. Pharmacol., 2014, 92, 506-516. 
    20. Sufang Wang, Qiyan Lv, Yu Yang*, Liang-Hong Guo*, Bin Wan, Lixia Zhao. Cellular target recognition of perfluoroalkyl acids: In vitro evaluation of inhibitory effects on lysine decarboxylase. Sci. Total Environ., 2014, 496, 381-388.
    21. Lixia Zhao*, Fanglan Geng, Fan Di, Liang-Hong Guo*, Bin Wan, Yu Yang, Hui Zhang, Guozhu Sun, Polyamine-functionalized carbon nanodots:a novel chemiluminescence probe for selective detection of iron (III) ions, RSC Advances, 2014, 4(86), 45768-45771.
    22. Dabin Wang, Lixia Zhao*, Liang-Hong Guo*, Hui Zhang, Online Detection of Reactive Oxygen Species in UV-irradiated Nano-TiO2 Suspensions by Continuous Flow Chemiluminescence, Anal. Chem., 2014, 86(21), 10533-10539.
    23. Qiang Ma*, Hua Bai, Chao Wang, Guang-Cheng Xi, Qing Zhang, Xian-Shuang Meng, Yun-Xia Chen, Jing-Rui Li, Hui-Juan Ma, Liang-Hong Guo*, “Investigation of noncovalent interactions between hydroxylatedpolybrominated diphenyl ethers and bovine serum albumin usingelectrospray ionization-ion mobility-mass spectrometry”, International Journal of Mass Spectrometry, 2014, 357, 34-44.
    24. Lianying Zhang, Xiao-Min Ren, and Liang-Hong Guo*, “Structure-Based Investigation on the Interaction of Perfluorinated Compounds with Human Liver Fatty Acid Binding Protein”, Environ. Sci. Technol. 2013, 47, 11293−11301.
    25. 李敏杰, 吴一萍, 张斌田, 郭良宏*, “DNA 脱碱基位点的检测与应用”, 《分析化学》,2013, 7, 965-972.
    26. Jing Sun, Hui Zhang,* Liang-Hong Guo,* Lixia Zhao. Two-Dimensional Interface Engineering of a Titania−Graphene Nanosheet Composite for Improved Photocatalytic Activity. ACS Applied Materials & Interfaces, 2013, 5: 13035−13041.
    27. Yiping Wu, Bintian Zhang, Liang-Hong Guo*, “Label-Free and Selective Photoelectrochemical Detection of Chemical DNA Methylation Damage Using DNA Repair Enzymes”, Analytical Chemistry, 2013, 85, 6908−6914.
    28. P.-X. Dong, B. Wan*, Z.-X. Wang, L.-H. Guo*, Y. Yang, L. Zhao, “Exposure of single-walled carbon nanotubes impairs the functions of primarily cultured murine peritoneal macrophages”, Nanotoxicology, 2013, 7, 1028-1042.
    29. Wan, B*.; Wang Z. X.; Lv, Q. Y.; Dong, P. X.; Zhao, LX; Yang, Y; Guo, L. H*., “Single-walled carbon nanotubes and graphene oxides induceautophagosome accumulation and lysosome impairment in primarilycultured murine peritoneal macrophages”, Toxicology Letters, 2013, 221, 118-127.
    30. Li, X. X.; Gao, Y.; Guo, L. H.*; Jiang, G. B., “Structure-dependent activities of hydroxylated polybrominated diphenyl ethers on human estrogen receptor”, Toxicology, 2013, 309, 15-22.
    31. Zhao, LX.*; Di, F.; Wang, DB.; Guo, L. H.*; Yang, Y.; Wan, B.; Zhang, H., “Chemiluminescence of carbon dots under strong alkaline solutions: a novel insight into carbon dot optical properties”, Nanoscale, 2013, 5, 2655-2658.
    32. Ren, X. M.; Guo, L. H.*, “Molecular toxicology of polybrominated diphenyl ethers: nuclear hormone receptor mediated pathways” Environmental Science: Processes & Impacts, 2013, 15, 702-708.
    33. Ren, X.M.; Guo, L. H.*; Gao, Y.; Zhang, B. T.; Wan, B., “Hydroxylated polybrominated diphenyl ethers exhibit different activities on thyroid hormone receptors depending on their degree of bromination”, Toxicology and Applied Pharmacology, 2013, 268, 256-263.
    34. Y. Gao, X. Li, L.-H. Guo*, “Assessment of Estrogenic Effects of Perfluoroalkyl Acids by Monitoring Ligand-induced Conformation State of Human Estrogen Receptor”, Environmental Science & Technology, 2013, 47, 634-641.
    35. Y. Gao, L.-H. Guo*, “Development of a Label-free Competitive Ligand Binding Assay with Human Serum Albumin on Molecularly Engineered Surface Plasmon Resonance Sensor Chip”, Analytical Methods, 2012, 4, 3718-3723.
    36. M.-Y. Wei, L.-H. Guo*, P. Famouri, “Electrocatalytic oxidation of tyrosines shows signal enhancement in label-free protein biosensors”, TRAC-Trends in Analytical Chemistry, 2012, 39, 130-14
    37. Z. Fan, Y. S. Keum, Q. X. Li, W. L. Shelver, L.-H. Guo*, “Development of indirect competitive fluorescence immunoassay for 2,2’,4,4’-tetrabromodiphenyl ether using DNA/dye conjugate as antibody multiple labels”, Journal of Environmental Science, 2012, 24, 1334-1340.
    38. B. Zhang, L.-H. Guo*, M. Greenburg*, “Quantification of 8-OxodGuo Lesions in Double-Stranded DNA Using a Photoelectrochemical DNA Sensor”, Analytical Chemistry, 2012, 84, 6048−6053
    39. B. Zhang, L.-H. Guo*, “Highly sensitive and selective photoelectrochemical DNA sensor for the detection of Hg2+ in aqueous solutions”, Biosensor & Bioelectronics, 2012, 37, 112–115.
    40. X. M. Ren, L.-H. Guo*, “Assessment of the Binding of Hydroxylated Polybrominated Diphenyl Ethers to Thyroid Hormone Transport Proteins Using a Site-Specific Fluorescence Probe”, Environmental Science & Technology, 2012, 46, 4633−4640.
    41. P. X. Dong, B. Wan*, L.-H. Guo, “In vitro toxicity of acid-functionalized single-walled carbon nanotubes: effects on murine macrophages and gene expression profiling”, Nanotoxicology, 2012, 6, 288-303.
    42. Z. Fan, Y. S. Keum, Q. X. Li, W. L. Shelver, L.-H. Guo*, “Sensitive immunoassay detection of multiple environmental chemicals on protein microarrays using DNA/dye conjugate as a fluorescent label”, Journal of Environmental Monitoring, 2012, 14, 1345–1352.
    43. Q. Ma∗, G.-C. Xi, C. Wang, H. Bai, Q. Zhang, H.-W. Xi, L.-H. Guo*, “Comprehensive two-dimensional separation for the analysis of alkylphenol ethoxylates employing hydrophilic interaction chromatography coupled with ion mobility-mass spectrometry”, International Journal of Mass Spectrometry, 2012, 315, 31– 39.
    44. Y. Liu, S. Jia, L.-H. Guo*, “Development of microplate-based photoelectrochemical DNA biosensor array for high throughput detection of DNA damage”, Sensors and Actuators B, 2012, 161, 334– 340.
    45. M.-Y. Wei, R. Huang, L.-H. Guo*, “High catalytic activity of indium tin oxide nanoparticle modified electrode towards electro-oxidation of ascorbic acid”, Journal of Electroanalytical Chemistry, 2012, 664, 156–160.
    46. J. Cao, L.-H. Guo*, B. Wan, Y. Wei, “In vitro fluorescence displacement investigation of thyroxine transport disruption by bisphenol A”, Journal of Environmental Sciences, 2011, 23, 315–321.
    47. Y. Yang, L.-H. Guo∗, N. Qu, M.-Y. Wei, L.-X. Zhao, B. Wan, “Label-free electrochemical measurement of protein tyrosine kinase activity and inhibition based on electro-catalyzed tyrosine signaling”, Biosensors and Bioelectronics, 2011, 28, 284– 290.
    48. Q. Ma, C. Wang, H. Bai, H.-W. Xi, G.-C. Xi, X.-M. Ren, Y. Yang, L.-H. Guo*, “Comprehensive Two-Dimensional Separation of Hydroxylated Polybrominated Diphenyl Ethers by Ultra-Performance Liquid Chromatography Coupled with Ion Mobility-Mass Spectrometry”, Journal of the American Society for Mass Spectrometry, 2011, 22, 1854–1861.
    49. Q. Zhang, S. Zhu, L.-H. Guo*, “DNA/Dye conjugates as multiple labels for antibodies in sensitive fluorescence immunoassays” In Bioconjugation Protocols: Strategies and Methods (2nd edition), Ed. S. S. Mark, Humana Press, 2011, pp 43-54.
    50. B. Zhang, X. Du, S. Jia, J. He, L.-H. Guo*, “Rapid assessment of DNA damage induced by polystyrene nanosphere suspension using a photoelectrochemical DNA sensor”, Science China: Chemistry, 2011, 54, 1260–1265.
    51. R. Huang, M.-Y. Wei, L.-H. Guo*, “Enhanced electrogenerated chemiluminescence of Ru(bpy)32+ /tripropylamine system on indium tin oxide nanoparticle modified transparent electrode”, Journal of Electroanalytical Chemistry, 2011, 656, 136–139.
    52. M.-Y. Wei∗, L.-H. Guo*, P. Famouri, “DNA biosensors based on metallo-intercalator probes and electrocatalytic amplification” Microchim Acta, 2011, 172, 247–260.
    53. N. Qu, L.-H. Guo∗, B.-Z. Zhu*, “An electrochemical biosensor for the detection of tyrosine oxidation induced by Fenton reaction”, Biosensors and Bioelectronics, 2011, 26, 2292–2296.
    54. 万斌*,郭良宏, “多溴联苯醚的环境毒理研究进展”, 《环境化学》, 2011, 30, 143-152.
    55. Yin Wei*, Yuan Lin, Ai-Qian Zhang∗, Liang-Hong Guo, Jie Cao, “Evaluation of the noncovalent binding interactions between polycyclic aromatic hydrocarbon metabolites and human p53 cDNA”, Science of the Total Environment, 2010, 408, 6285–6290.
    56. J. Cao, Y. Lin, L.-H. Guo∗, A.-Q. Zhang∗, Y. Wei, Y. Yang, “Structure-based investigation on the binding interaction of hydroxylated polybrominated diphenyl ethers with thyroxine transport proteins”, Toxicology, 2010, 277, 20–28.
    57. R. Huang, L.-R. Wang, L.-H. Guo∗, “Highly sensitive electrochemiluminescence displacement method for the study of DNA/small molecule binding interactions”, Anal. Chim. Acta. 2010, 676, 41–45.
    58. R. Huang, L.-H. Guo*, “Lack of nano size effect on electrochemistry of dopamine at a gold nanoparticle modified indium tin oxide electrode”, Sci. China Chem., 2010, 53, 1778–1783.
    59. S. Jia, B.-Z. Zhu, L.-H. Guo*, “Detection and mechanistic investigation of halogenated benzoquinone induced DNA damage by photoelectrochemical DNA sensor”, Anal. Bioanal. Chem. 2010, 397, 2395–2400.
    60. Y. Yang, L. Zhao*, “Sensitive fluorescent sensing for DNA assay”, Trends in Analytical Chemistry, 2010, 29, 980-1003.
    61. L. Zhao,* L. Sun, X. Chu, “Chemiluminescence immunoassay”, Trends in Analytical Chemistry, 2009, 28, 404-415.  
    62. 杨郁, 郭良宏*, “超分子主体化合物环糊精应用于环境污染物分离分析的研究进展”, 《科学通报》, 2009, 54, 128-137.
    63. L-R. Wang, Y. Wang, J.-W. Chen*, L.-H. Guo*, “A structure-based investigation on the binding interaction of hydroxylated polycyclic aromatic hydrocarbons with DNA”, Toxicology, 2009, 262, 250–257.
    64. Q. Zhang, R. Huang, L.-H. Guo*, “One-step and high-density protein immobilization on epoxysilane-modified silica nanoparticles”, Chinese Sci Bull, 2009, 54, 2620-2626.
    65. M.-Y. Wei, S.-D. Wen, X.-Q. Yang, L.-H. Guo*, “Development of redox-labeled electrochemical immunoassay for polycyclic aromatic hydrocarbons with controlled surface modification and catalytic voltammetric detection”, Biosensors and Bioelectronics, 2009, 24, 2909–2914.
    66. Y. Wei, L.-H. Guo*, “Binding interaction between polycyclic aromatic compounds and DNA by fluorescence displacement method”, Environmental Toxicology and Chemistry, 2009, 28, 940–945.
    67. 韦明元,郭良宏*, “环境污染物的免疫传感检测方法进展’’, 《化学进展》, 2009, 21, 492-502.
    68. X.-Q. Yang, L.-H. Guo*, “Enhanced Electrochemical Activity of Redox-labels in Multi-layered Protein Films on Indium Tin Oxide Nanoparticle-based Electrode”, Analytica Chimica Acta, 2009, 632, 15–20.
    69. Y. Chen, L.-H. Guo*, “Combined fluorescence and electrochemical investigation on the binding interaction between organic acid and human serum albumin”, Journal of Environmental Sciences, 2009, 21, 373–379.
    70. M. Liang, L.-H. Guo*, “Application of nanomaterials in environmental analysis and monitoring”, Journal of Nanoscience and Nanotechnology, 2009, 9, 2283–2289.
    71. Y. Chen, L.-H. Guo*, “Fluorescence study on site-specific binding of perfluoroalkyl acids to human serum albumin” Archives of Toxicology. 2009, 83, 255–261.

    Supported Projects

    1. National natural science of China: Dynamic monitoring of intermediates and mechanism involved the photocatalytic degradation of halophenols and its toxicity risk assessment based on chemiluminescence. 2017.01-2020.12, Chair
    2. Key Research Program of Frontier Sciences,CAS: Noval mechanisms for the estrogenic effects of polybrominated diphenyl ethers and their metabolites. 2016.08-2020.12, Chair
    3. National natural science of China: Environmental transformation behavior and toxicity of nano-TiO2 in aquatic system under sunlight irradiation. 2016.01-2019.12, Chair
    4. Major project of National natural science of China: Studies on the Molecular Mechanisms of PM2.5 Oxidative Stress Using Particle Models. 2016.01-2019.12, Chair
    5. National natural science of China: Study on perfluorinated compounds recognition for new target-protein tyrosine phosphatase and molecular mechanism of toxicity. 2016.01-2019.12, Chair
    6. National natural science of China: The toxicological effects of carbon nanoparticles on cell autophagy and the toxic mechanisms. 2015.01-2018.12, Chair
    7. National natural science of China: Study on the endocrine disrupting effects and molecular mechanisms of perfluorinated compounds. 2015.01-2017.12, Chair
    8. Strategic pilot project of the Chinese Academy of Sciences B: Study on the endocrine disrupting and developmental toxicity of typical pollutants.  2014.07-2019.06, Chair
    9. National natural science of China: Study on the electrochemiluminescence assay for DNA damage and applications. 2014.01-2017.12,Chair
    10. National natural science of China: Study for rapid screening of persistant toxic substance's disrupting effect on the reversible protein glycosylation and related mechanism.2014.01-2017.12, Chair
    11. National natural science of China: Study on the bioavailablity, biodegradation and toxicity of single-walled carbon nanotubes. 2013.01-2016.12, Chair
    12. National natural science of China: Graphene based Z-scheme photocatalyst systems for photocatalytic debromination of PBDEs. 2013.01-2015.12, Chair
    13. National natural science of China: Singlet oxygen channeling homogenous chemiluminescence immunoassay enhanced by nanoparticles and its analytical application for environmental estrogens. 2012.01-2015.12, Chair
    14. National natural science of China: Real-time dynamic and high-throughput study on the toxic effects of dioxins on amino acid decarboxylase based on supramolecular system. 2011.01-2013.12, Chair
    15. National 973 project:Fabrication of nanomaterials-based devices for water treatment and their applications. 2010.10-2015.08, Chair
    16. National natural science of China: Detection of Protein Phosphorylation Based on Tyrosine Electrochemistry. 2009.01-2011.12, Chair
    17. National Natural Science Funds for Distinguished Young Scholar: bio-analytical chemistry. 2009.01-2012.12, Chair
    18. Major project of National natural science of China: Bioaccumulation and metabolism of typical POPs and molecular interactions. 2009.01-2012.12, Chair
    19. National natural science of China: Study on the immunotoxicity of single-walled carbon nanotubes and the toxicogenomic analysis on the toxic mechanisms. 2009.01-2011.12, Chair
    20. National natural science of China: Study of chemiluminescence behavior and mechanism for the degradation of organochlorines involved nanoparticles. 2009.01-2011.12, Chair
    21. Key Programs of the Chinese Academy of Sciences for research equipments: Instrument for rapid detection of environmental genotoxic substances. 2008.03-2010.06
    22. Hundred-Talent Program of Chinese Academy of Sciences: Bio-analysis and Ecotoxicology. 2004.06-2007.05 , Chair


    1. 中国科学院“百人计划”(2004)
    2. 中国科学院院长优秀奖 (2007)
    3. 国家自然科学基金委杰出青年基金(2009)
    4. 中国科学院优秀研究生指导教师(2009)
    5. 中国科学院优秀博士学位论文奖(2009)
    6. 中国科学院杰出科技成就奖(2013)
    7. 国家百千万人才工程“有突出贡献中青年专家” (2013)